Bacillus amyloliquefaciens WF2020 isolated from fermented pickles promotes longevity and health in Caenorhabditis elegans via JNK and p38 MAPK pathways.
Abstract — Entities Highlighted
Gene/ProteinChemicalDiseasePhenotypeAnatomyDev. Stage
Bacillus amyloliquefaciens, a potential probiotic for use in food and feed production, can exert anti-aging effects in a strain-specific manner. However, the molecular mechanisms underlying its anti-aging effects remain poorly understood. This study explored the effects of B. amyloliquefaciens WF2020 (WF2020), isolated from Chinese fermented pickles, on longevity and health and investigated the underlying mechanisms in Caenorhabditis elegans. Interestingly, WF2020 was found to increase mean lifespan, worm length, body bends, and resistance to heat, oxidative stress, and Staphylococcus aureus infection. It also improved mitochondrial transmembrane potential and antioxidative status, reduced lipid accumulation and oxidative damage, and altered the expression of several genes involved in cell apoptosis, fat metabolism, host tolerance to heat and oxidative stress, and immune responses, compared to Escherichia coli OP50 (OP50), a standard food source for C. elegans. Moreover, WF2020-fed loss-of-function mutants for nsy-1, sek-1, pmk-1, jkk-1, jnk-1, daf-16, and hsf-1 did not exhibit lifespan extension. Additionally, WF2020-fed pmk-1 and jnk-1 mutants showed similar worm length, body bends, lipid accumulation, mitochondrial transmembrane potential, and antioxidative properties to the OP50 group. Correspondingly, WF2020 significantly upregulated the expression of nsy-1, sek-1, pmk-1, jkk-1, and jnk-1 and increased the proportion of DAF-16::GFP in the nucleus, along with the expression of HSP-16.2::GFP. In conclusion, WF2020 activated the p38 MAPK and JNK pathways to regulate the functions of HSF-1 and DAF-16, thereby promoting longevity and health in C. elegans. These findings suggest that WF2020 could be a potential probiotic or a starter for use in food and feed production to delay aging and promote health.
Relation Network
Specialists Used
deepseek: 44gene_gene: 8gene_expression: 2gene_phenotype: 2
All Extracted Relations (57)
| Entity 1 | Relation | Entity 2 | Confidence | Specialist | Direction |
|---|---|---|---|---|---|
| jkk-1 | regulation | HSP-16.2 | 100% | gene_gene | supports |
| nsy-1 | regulation | DAF-16 | 100% | gene_gene | supports |
| HSF-1 | regulation | DAF-16 | 100% | gene_gene | supports |
| nsy-1 | regulation | pmk-1 | 100% | gene_gene | supports |
| pmk-1 | regulation | jnk-1 | 100% | gene_gene | supports |
| nsy-1 | regulation | daf-16 | 100% | gene_gene | supports |
| jnk-1 | regulation | DAF-16 | 100% | gene_gene | supports |
| DAF-16 | expressed in | nucleus | 100% | gene_expression | supports |
| jkk-1 | expressed in | nucleus | 100% | gene_expression | supports |
| sek-1 | regulation | jnk-1 | 100% | gene_gene | supports |
| jnk-1 | causes phenotype | long | 92% | gene_phenotype | supports |
| HSF-1 | causes phenotype | long | 92% | gene_phenotype | supports |
| Bacillus amyloliquefaciens WF2020 | positively regulates | sek-1 | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | positively regulates | pmk-1 | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | positively regulates | jkk-1 | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | positively regulates | jnk-1 | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | positively regulates | DAF-16 | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | positively regulates | HSP-16.2 | 85% | deepseek | supports |
| nsy-1 | positively regulates | longevity | 85% | deepseek | supports |
| sek-1 | positively regulates | longevity | 85% | deepseek | supports |
| pmk-1 | positively regulates | longevity | 85% | deepseek | supports |
| jkk-1 | positively regulates | longevity | 85% | deepseek | supports |
| jnk-1 | positively regulates | longevity | 85% | deepseek | supports |
| daf-16 | positively regulates | longevity | 85% | deepseek | supports |
| hsf-1 | positively regulates | longevity | 85% | deepseek | supports |
| pmk-1 | positively regulates | worm length | 85% | deepseek | supports |
| jnk-1 | positively regulates | worm length | 85% | deepseek | supports |
| pmk-1 | positively regulates | body bends | 85% | deepseek | supports |
| jnk-1 | positively regulates | body bends | 85% | deepseek | supports |
| pmk-1 | negatively regulates | lipid accumulation | 85% | deepseek | refutes |
| jnk-1 | negatively regulates | lipid accumulation | 85% | deepseek | refutes |
| pmk-1 | positively regulates | mitochondrial transmembrane potential | 85% | deepseek | supports |
| jnk-1 | positively regulates | mitochondrial transmembrane potential | 85% | deepseek | supports |
| pmk-1 | positively regulates | antioxidative properties | 85% | deepseek | supports |
| jnk-1 | positively regulates | antioxidative properties | 85% | deepseek | supports |
| p38 MAPK pathway | regulates | HSF-1 | 85% | deepseek | supports |
| JNK pathway | regulates | HSF-1 | 85% | deepseek | supports |
| p38 MAPK pathway | regulates | DAF-16 | 85% | deepseek | supports |
| JNK pathway | regulates | DAF-16 | 85% | deepseek | supports |
| HSF-1 | positively regulates | health | 85% | deepseek | supports |
| DAF-16 | positively regulates | health | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | positively regulates | p38 MAPK pathway | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | positively regulates | JNK pathway | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | positively regulates | health | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | negatively regulates | aging | 85% | deepseek | refutes |
| Bacillus amyloliquefaciens WF2020 | positively regulates | longevity | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | positively regulates | worm length | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | positively regulates | body bends | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | positively regulates | heat resistance | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | positively regulates | oxidative stress resistance | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | positively regulates | Staphylococcus aureus infection resistance | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | positively regulates | mitochondrial transmembrane potential | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | positively regulates | antioxidative status | 85% | deepseek | supports |
| Bacillus amyloliquefaciens WF2020 | negatively regulates | lipid accumulation | 85% | deepseek | refutes |
| Bacillus amyloliquefaciens WF2020 | negatively regulates | oxidative damage | 85% | deepseek | refutes |
| Bacillus amyloliquefaciens WF2020 | positively regulates | nsy-1 | 85% | deepseek | supports |
| HSF-1 | regulates | DAF-16 | 56% | — | supports |